Department: 
Anatomical Pathology
Laboratory: 
Histology/Immunohistochemistry
Tube Colour: 
Yellow top universal
Pseudonyms: 
IF, DIF
Test Code: 
HIST
Availability: 
Week days. Specimens taken out of hours are stored at 4̊ C
Collection Requirements: 
Fresh tissue is placed on saline soaked gauze. Do not allow the tissue to dry out. Refrigerate/ send to Pathology CSR as soon as possible. Specimens taken out of hours are stored at 4̊ C. Usually (not always) accompanied by a specimen in formalin for histopathology. If histopathology is required, collect separate formalin fixed sample. If in doubt, contact the ‘on-call’ pathologist in Anatomical Pathology.
Requires own tube?: 
No
Fee: 
Medicare rebatable
Notes: 
Immunofluorescence staining involves staining frozen sections of fresh (unfixed) biopsy samples. Optimum results are obtained by minimizing the time interval between excision of the biopsy and freezing the specimen for sectioning. Specimens commonly received for IF: Skin punch biopsy for immunobullous disorders or vasculitis Buccal mucosa biopsy for immunobullous disorders Renal biopsy if anti-Glomerular Basement Membrane disease is a possibility