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Pathology handbook

Acquired JAK2 (Val617Phe) Mutation

Test Code: 
PJK14
Tube Colour: 
Lavender
Department: 
Haematology
Pseudonyms: 
JAK 2 Mutation; JAK2 Kinase Mutation Analysis
Collection Requirements: 
3 - 10 ml EDTA Whole Blood or 1-3mL Bone Marrow
Reference Range: 
Availability: 
1-2 weeks to result
Requires own tube?: 
Yes
Internal Spec Code: 
EW, BM
Fee: 
Contact reference laboratory directly for up to date fee advice.
Reference Laboratory: 
Peter MacCallum Cancer CentrePathology Department Level 4305 Grattan StreetMelbourne, Vic 3000
Laboratory: 
Molecular Haematology
Specimen MUST be delivered to laboratory within 24 hours of collection (Mon - 3pm Fri, excluding public holidays)

ADAMTS-13

Test Code: 
ATS13
Tube Colour: 
Light Blue
Department: 
Haematology
Pseudonyms: 
ADAMTS 13; ATS13
Availability: 
Weekly (routine); Same day (URGENT)
Requires own tube?: 
No
Fee: 
Routine: AUD $185 not Medicare rebatable; URGENT: AUD $350 not Medicare rebatable
Reference Laboratory: 
Monash Medical Centre
Monash Health Pathology
246 Clayton Road
Clayton

Alpha 2 Antiplasmin

Test Code: 
MISCH
Department: 
Haematology
Pseudonyms: 
Collection Requirements: 
Contact Pathology (Haematology) if collection requirement details are required
Reference Range: 
As reported
Availability: 
Requires own tube?: 
Yes
Internal Spec Code: 
C
Fee: 
Contact reference laboratory directly for up to date fee advice.
Reference Laboratory: 
Monash Medical CentreMonash Health Pathology246 Clayton RoadClayton, Vic 3168tel 03 9594 4538
Laboratory: 
Haematology

Anti-Human PML Prot.

Test Code: 
MISCH
Tube Colour: 
Lavender
Department: 
Haematology
Pseudonyms: 
Collection Requirements: 
3-10mL EDTA Blood or 1-3mL Bone Marrow
Reference Range: 
Availability: 
2-3 weeks to result
Requires own tube?: 
Yes
Internal Spec Code: 
EW, BM
Fee: 
Contact reference laboratory directly for up to date fee advice.
Reference Laboratory: 
Peter MacCallum Cancer CentrePathology Department Level 4305 Grattan StreetMelbourne, Vic 3000
Laboratory: 
Molecular Haematology
Specimen MUST be delivered to laboratory within 24 hours of collection (Mon - 3pm Fri, excluding public holidays)

Antibody Titre (RMH only)

Test Code: 
Titre
Department: 
Haematology
Pseudonyms: 
Anti-A, Anti-B
Collection Requirements: 
6mL EDTA tube (Blood Bank) + 2 x 6mL Serum (GEL FREE) - all tubes must include date, time and collector signature
Requires own tube?: 
Yes
Laboratory: 
Blood Transfusion
1. SPECIMENS WILL NOT BE ACCEPTED FOR PRETRANSFUSION TESTING UNLESS: i) the specimen and request slip conform to the Blood Transfusion protocols of the relevent site. If Bradma labels are used they must be signed and dated to confirm the patient ID has been checked. ii) The specimen collection declaration is completed by the person collecting and labelling the specimen. Please label the request slip with the ward and clinical details. 2. IF URGENT, TELEPHONE THE LABORATORY. 3. Use "Transfusion Test/ Product Request Form".

Antithrombin

Test Code: 
AT
Tube Colour: 
Light Blue
Department: 
Haematology
Pseudonyms: 
Anti-Thrombin III (AT, AT3, ATIII)
Collection Requirements: 
Sodium Citrate Tube (Coagulation)
Reference Range: 
80- 120% Functional
Availability: 
24 hours
Requires own tube?: 
No
Internal Spec Code: 
C
Fee: 
Medicare rebatable in a patient with a history of venous thromboembolism OR in a first degree relative of a person who has a proven defect of antithrombin III, protein C, protein S or activated protein C resistance.
Laboratory: 
Haematology

APC Resistance Screen

Test Code: 
APCR
Tube Colour: 
Light Blue
Department: 
Haematology
Pseudonyms: 
Activated Protein C Resistance_x000D__x000D_(APC Resistance, APCR)
Collection Requirements: 
Sodium Citrate Tube (Coagulation)
Reference Range: 
Normal Ratio > 0.86
Availability: 
Fortnightly (unless by prior arrangement)
Requires own tube?: 
No
Internal Spec Code: 
C
Fee: 
Medicare rebatable in a patient with a history of venous thromboembolism OR in a first degree relative of a person who has a proven defect of antithrombin III, protein C, protein S or activated protein C resistance.
Laboratory: 
Haematology
See also Hypercoagulation screen.

APTT

Test Code: 
APTT
Tube Colour: 
Light Blue
Department: 
Haematology
Pseudonyms: 
Activated Partial Thromboplastin Time (APTT)
Collection Requirements: 
Sodium Citrate Tube (Coagulation)
Reference Range: 
Normal: 24- 36 sec _x000D_onds
Availability: 
24 hours
Requires own tube?: 
No
Internal Spec Code: 
C
Fee: 
Medicare rebatable
Laboratory: 
Haematology
See also coagulation profile, DIC screen * Please request specific test(s) and specify any anticoagulation"

Blood Group Only

Test Code: 
Grp
Tube Colour: 
Pink
Department: 
Haematology
Pseudonyms: 
Blood Group (ABO & Rh(D) group)
Collection Requirements: 
6mL EDTA tube (Blood Bank)
Reference Range: 
Availability: 
Daily
Requires own tube?: 
Yes
Internal Spec Code: 
EB
Fee: 
Medicare rebatable
Laboratory: 
Blood Transfusion
1. SPECIMENS WILL NOT BE ACCEPTED FOR PRETRANSFUSION TESTING UNLESS: i) the specimen and request slip conform to the Blood Transfusion protocols of the relevent site. If Bradma labels are used they must be signed and dated to confirm the patient ID has been checked. ii) The specimen collection declaration is completed by the person collecting and labelling the specimen. Please label the request slip with the ward and clinical details. 2. IF URGENT, TELEPHONE THE LABORATORY. 3. Use "Transfusion Test/ Product Request Form".

Bone Marrow (Aspirate and Trephine)

Test Code: 
BMAT
Department: 
Haematology
Pseudonyms: 
Bone Marrow Biopsy (Aspirate and Trephine)
Collection Requirements: 
By arrangement, contact Haem. Registrar (93427402)
Reference Range: 
Availability: 
By arrangement
Requires own tube?: 
Yes
Internal Spec Code: 
Fee: 
Medicare rebatable
Laboratory: 
Haematology
By arrangement, contact Haem. Registrar (93427402) Verbal report on aspirate on the same day if significant.

Bone Marrow Aspirate

Test Code: 
Asp
Department: 
Haematology
Pseudonyms: 
Bone Marrow (Aspirate)
Collection Requirements: 
By arrangement, contact Haem. Registrar (93427402)
Reference Range: 
Availability: 
By arrangement
Requires own tube?: 
Yes
Internal Spec Code: 
Fee: 
Medicare rebatable
Laboratory: 
Haematology
By arrangement, contact Haem. Registrar (93427402) Verbal report on aspirate on the same day if significant.

c-kit Gene (D816V) Mutation

Test Code: 
PMASTO
Tube Colour: 
Lavender
Department: 
Haematology
Pseudonyms: 
c-KIT mutation analysis for Mastocytosis
Collection Requirements: 
3-10mL EDTA Whole Blood or 1-3mL Bone Marrow
Reference Range: 
Availability: 
2-3 weeks to result
Requires own tube?: 
Yes
Internal Spec Code: 
EW, BM
Fee: 
Contact reference laboratory directly for up to date fee advice.
Reference Laboratory: 
Peter MacCallum Cancer CentrePathology Department Level 4305 Grattan StreetMelbourne, Vic 3000
Laboratory: 
Molecular Haematology
Specimen MUST be delivered to laboratory within 24 hours of collection (Mon - 3pm Fri, excluding public holidays)

CD34 Counting

Test Code: 
CD34
Department: 
Haematology
Pseudonyms: 
Collection Requirements: 
Contact Pathology (Haematology) if collection requirement details are required
Reference Range: 
Availability: 
By arrangement
Requires own tube?: 
Yes
Internal Spec Code: 
Fee: 
Medicare rebatable
Laboratory: 
Flow Cytometry

CEBPA Mutation

Test Code: 
PCEBPA
Tube Colour: 
Lavender
Department: 
Haematology
Pseudonyms: 
C/EBPA; CCAAT/Enhancer Binding Protein Alpha
Collection Requirements: 
3-10mL EDTA Whole Blood or 1-3mL Bone Marrow
Reference Range: 
Availability: 
2-3 weeks to result
Requires own tube?: 
Yes
Internal Spec Code: 
EW, BM
Fee: 
Contact reference laboratory directly for up to date fee advice.
Reference Laboratory: 
Peter MacCallum Cancer CentrePathology Department Level 4305 Grattan StreetMelbourne, Vic 3000
Laboratory: 
Molecular Haematology
Specimen MUST be delivered to laboratory within 24 hours of collection (Mon - 3pm Fri, excluding public holidays)

Coagulation Profile | (Prothrombin Time, INR, APTT, Fibrinogen)

Test Code: 
COAG
Tube Colour: 
Light Blue
Department: 
Haematology
Pseudonyms: 
Coagulation Profile, Clotting Screen
Collection Requirements: 
Sodium Citrate Tube (Coagulation)
Reference Range: 
As reported
Availability: 
24 hours
Requires own tube?: 
Yes
Internal Spec Code: 
C
Fee: 
Medicare rebatable
Laboratory: 
Haematology
* Please request specific test(s) and specify any anticoagulation Tests include: Prothrombin Time (PT) Partial Thromboplastin Time (APTT) Fibrinogen

Cold Agglutinins Screen

Test Code: 
CAGGS
Tube Colour: 
Red
Department: 
Haematology
Pseudonyms: 
Cold Agglutinins
Collection Requirements: 
SEE NOTES 2 x 6mL Serum (Clot, No Gel) specimen must be kept at 37oC from collection to the Laboratory. at RMH, thermos for transport available from lab.
Reference Range: 
Titre
Availability: 
Daily (Mon - Fri)
Requires own tube?: 
No
Internal Spec Code: 
SB
Fee: 
Medicare rebatable
Laboratory: 
Blood Transfusion
Specimen must be kept at 37oC from collection to the Laboratory. Contact Laboratory before collection: at RMH, Thermos for transport available from lab. Sign, date and time specimens and request form.

Collagen Bind Assay

Test Code: 
CBA
Tube Colour: 
Light Blue
Department: 
Haematology
Pseudonyms: 
Collagen Binding Assay
Collection Requirements: 
Sodium Citrate Tube (Coagulation)
Reference Range: 
50 - 200%
Availability: 
Every 2-3 weeks
Requires own tube?: 
Yes
Internal Spec Code: 
C
Fee: 
Contact reference laboratory directly for up to date fee advice.
Reference Laboratory: 
Alfred Pathology Services55 Commercial RoadPrahan 3181tel 03 9076 3888
Laboratory: 
Haematology

Coombs / DAT.

Test Code: 
DAT
Tube Colour: 
Pink
Lavender
Department: 
Haematology
Pseudonyms: 
Coomb's Test (Direct Antiglobulin Test, DAT)
Collection Requirements: 
6mL EDTA tube (Blood Bank) or 3ml EDTA
Reference Range: 
Negative
Availability: 
Daily
Requires own tube?: 
No
Internal Spec Code: 
EW or EB
Fee: 
Medicare rebatable
Laboratory: 
Blood Transfusion

Cross-match (X-match)

Test Code: 
XM# (where # = no. of units orderred)
Tube Colour: 
Pink
Department: 
Haematology
Pseudonyms: 
Collection Requirements: 
6mL EDTA tube (Blood Bank)
Reference Range: 
Availability: 
Requires own tube?: 
Yes
Internal Spec Code: 
EB
Fee: 
Medicare rebatable
Laboratory: 
Blood Transfusion
1. SPECIMENS WILL NOT BE ACCEPTED FOR PRETRANSFUSION TESTING UNLESS: i) the specimen and request slip conform to the Blood Transfusion protocols of the relevent site. If Bradma labels are used they must be signed and dated to confirm the patient ID has been checked. ii) The specimen collection declaration is completed by the person collecting and labelling the specimen. Please label the request slip with the ward and clinical details. 2. IF URGENT, TELEPHONE THE LABORATORY. 3. Use "Transfusion Test/ Product Request Form".

Cryopreservation

Test Code: 
CRYO
Department: 
Haematology
Pseudonyms: 
Collection Requirements: 
Contact Pathology (Haematology) if collection requirement details are required
Reference Range: 
Availability: 
By arrangement
Requires own tube?: 
Yes
Internal Spec Code: 
Fee: 
Laboratory: 
Blood Transfusion

Cytogenetics

Test Code: 
CGENS
Department: 
Haematology
Pseudonyms: 
Cytogenetics
Collection Requirements: 
Lithium Heparin with no Gel
Reference Range: 
As reported
Availability: 
Requires own tube?: 
Yes
Internal Spec Code: 
Fee: 
Contact reference laboratory directly for up to date fee advice.
Reference Laboratory: 
St Vincents Hospital Fitzroy OR Royal Children's Hospital
Laboratory: 
Haematology

D-Dimer

Test Code: 
DD
Tube Colour: 
Light Blue
Department: 
Haematology
Pseudonyms: 
D-Dimer, D dimer, Cross linked Fibrin degradation products / FDP
Collection Requirements: 
Sodium Citrate Tube (Coagulation)
Reference Range: 
Normal < 0.50 mg/L (FEU)
Availability: 
24 hours
Requires own tube?: 
No
Internal Spec Code: 
C
Fee: 
Medicare rebatable
Laboratory: 
Haematology

DIC Screen | (Prothrombin Time, INR, APTT, Fibrinogen + D Dimer)

Test Code: 
DIC
Tube Colour: 
Light BlueANDLavender
Department: 
Haematology
Pseudonyms: 
D.I.C. Screen (DIC)
Collection Requirements: 
Sodium Citrate Tube (Coagulation) and EDTA tube (Platelet Count)
Reference Range: 
As reported
Availability: 
24 hours
Requires own tube?: 
Yes
Internal Spec Code: 
C, EW
Fee: 
Medicare rebatable
Laboratory: 
Haematology
Sodium Citrate and EDTA should be collected. This screen includes Prothrombin Time, INR, APTT, Fibrinogen + D Dimer and the EDTA collected is required for a Platelet Count

DNA for Thalassaemia

Test Code: 
DNAth
Tube Colour: 
Pink
Department: 
Haematology
Pseudonyms: 
DNA Studies for Thalassaemia
Collection Requirements: 
6mL EDTA tube
Reference Range: 
Availability: 
Requires own tube?: 
Yes
Internal Spec Code: 
Ew
Fee: 
Contact reference laboratory directly for up to date fee advice.
Reference Laboratory: 
Monash Medical CentreMonash Health Pathology246 Clayton RoadClayton, Vic 3168tel 03 9594 4538
Laboratory: 
Haematology
This test requires an FBE, Haemoglobin Electrophoresis and Iron studies to be requested in order to fully interpret results. Turn around time - up to 3 months or longer.

DNA Storage

Test Code: 
PSDNA
Tube Colour: 
Lavender
Department: 
Haematology
Pseudonyms: 
DNA Storage
Collection Requirements: 
3-10mL EDTA Whole Blood or 1-3mL Bone Marrow
Requires own tube?: 
Yes
Internal Spec Code: 
EW, BM
Fee: 
AUD $40 not Medicare rebatable
Reference Laboratory: 
Peter MacCallum Cancer CentrePathology Department Level 4305 Grattan StreetMelbourne, Vic 3000
Laboratory: 
Molecular Haematology
Specimen MUST be delivered to laboratory within 24 hours of collection (Mon - 3pm Fri, excluding public holidays)