Test Code: 
HIST
Tube Colour: 
Yellow top universal
Department: 
Anatomical Pathology
Pseudonyms: 
IF, DIF
Collection Requirements: 
Fresh tissue is placed on saline soaked gauze. Do not allow the tissue to dry out. Refrigerate/ send to Pathology CSR as soon as possible. Specimens taken out of hours are stored at 4̊ C. Usually (not always) accompanied by a specimen in formalin for histopathology. If histopathology is required, collect separate formalin fixed sample. If in doubt, contact the ‘on-call’ pathologist in Anatomical Pathology.
Availability: 
Week days. Specimens taken out of hours are stored at 4̊ C
Requires own tube?: 
No
Fee: 
Medicare rebatable
Laboratory: 
Histology/Immunohistochemistry
Immunofluorescence staining involves staining frozen sections of fresh (unfixed) biopsy samples. Optimum results are obtained by minimizing the time interval between excision of the biopsy and freezing the specimen for sectioning. Specimens commonly received for IF: Skin punch biopsy for immunobullous disorders or vasculitis Buccal mucosa biopsy for immunobullous disorders Renal biopsy if anti-Glomerular Basement Membrane disease is a possibility