Special Collection Requirements: SEE NOTES Serum - Plain Tube With No Gel. 3 Ml Minimum. Refer To Notes For Important Specimen Handling Requirements. Transport To CSR Urgently For Aliquoting
At least 3 ml of whole blood collected into a plain gel-free (neutral) serum tube.
The blood is allowed to clot at room temperature for up to 60 minutes
Centrifuge samples within 1 hour of collection, after clotting, for 10 min at 3800 rpm and 4oC.
Transfer cell-free supernatant to three 300uL aliquot tubes (Sarsdedt).
Store all the aliquots in a sample box at –70oC immediately, until transport
Serum samples must be transferred to the Immunology laboratory Royal Children’s Hospital on dry ice and not allowed to thaw during transport.
6 ml Serum with no Gel.
Send to pathology immediately for centrifugation and storage at -70C.
Requires own tube?:
Royal Children's Hospital
Mannose Binding Lectin is part of the Complement Functional panel. The Complement Functional Panel includes three tests : Classical pathway (CH50), Alternative pathway (AH50) and Mannose Binding Lectin.
6 mL Lithium heparin NO GEL ON ICE
- It is preferred that the patient be resting supine for 15 mins prior to collection. It should be noted on the request if the sample is fasting/non-fasting and supine/non-supine.
- The specimen is usually collected from an indwelling line.
- Samples should be immediately sent to the Laboratory on ice.
If results are moderately elevated (3 x upperlimit), it is recommended that a second analysis is performed with a 12 hr fasting specimen collected after rest (>15min) in the supine position as requirements. Consideration should also be given to medications, especially those with potential to alter levels of biogenic amines such as Aldomet, Tricyclic Antidepressants, MAO inhibitors and other adrenergic agonists or antagonists. Potential illnesses may also contribute to altered states of biogenic amines. Other biochemical evaluation such as 24hr urinary catecholamines or metanephrines should also be considered.
Persistant highly elevated levels may be associated with the presence of a neuro-endocrine tumour and the profile of excretion may relate to tumour type.